Department of Anatomy

Histology Laboratory

Department of Anatomy, JNMC, AMU

SOP:

• Wear appropriate PPE (face mask and lab coat).

• Follow standard precautions of handling microscopes and slides.

• Proper precautions of handling instruments and chemicals.

• Perform regular cleaning and maintenance of instruments, microscopes & slides.

• All the steps from procurement of tissue to mounting of slide are performed taking standard precautions.

Facilities:

1. Histology lab is used for taking histology practical of MBBS and BDS students.

2. Fixation of tissue (formalin, karnowsky’s fluid)

3. Paraffin embedding (block formation)

4. Section cutting (by microtome)

5. Hematoxylin and eosin stain

6. Special stains- Thionin, Van Gieson, Toluidine Blue, Alcian Blue, Giemsa, Reticulin, Nissl, Orcein, Sudan Black B, Masson's Trichrome, Mallory's Trichrome, Periodic Acid Schiff and Silver nitrate stain.

7. Mounting of slide

Staff:

1. Technical Assistant- Mrs. Farhana Naqvi

2. Lab Assistant- Mr. Mohd Anees Khan

3. MTS of the department clean the laboratory on regular basis.

Instruments:

Microscopes- dissecting, monocular, binocular and projection Slide projector Microtomes- Rotary and sliding

Tissue floatation bath/hot plate for flattering sections

Incubator 00-900

Electronic balance

Heating plate Staining sets Slide cabinets Hot sterilizer oven

Museum

Department of Anatomy, JNMC, AMU

SOP:

Museum consists of:

• 345 soft tissue specimens.

• 233 models.

They are arranged region wise:

1. Embryology

2. Thorax

3. Brain

4. Abdomen & Pelvis

5. Upper Limb

6. Lower Limb

7. Cross Sectional Anatomy

Gross anatomy specimens are kept in 10% formalin solution in glass jars. Each jar is labelled & tightly sealed. We have made description of each specimen in printed format along with their labelling.

We have allotted the gross anatomy specimens region wise to our teaching staff as follows:

Embryology	:	Prof. Farah Ghaus
Thorax	:	Dr. Nema Usman
Brain	:	Prof. Farah Ghaus
Abdomen & Pelvis	:	Dr. Mohd Imran
Upper & Lower Limb	:	Dr. Nema Usman
Cross Sectional Anatomy	:	Dr. Mohd Imran

They take care of each specimen by taking weekly visits.

Museum technical staff looks after the water level, position, color of water, leakage of jar. If the color of water is changed or leakage is present then he/she will immediately change the water. Alternate day cleaning is done with all hygienic measures.

Computer lab and Seminar library

Department of Anatomy, JNMC, AMU

• Department has a well-stocked Seminar Library and Computer lab.

• Library contains sufficient number of books, journals and thesis.

• Computer sets are used by faculty members, senior residents, MD and PhD students for academic and research purposes.

• Library books are catalogued with Accession numbers for each, in line with the procedure which is followed by the Maulana Azad Library of the University.

• Computers’ hardware and software are updated regularly.

SOP Seminar Library:

1. All users are required to sign the register kept with the designated staff appointed as caretaker.

2. Certain books like the Dissertation /Thesis cannot be taken outside the Department and should be returned the same day.

3. Faculty/JR/SR, who are issued the book are responsible for the proper maintenance of the book and should return the same in the condition it was issued.

4. In case of damage or loss of the book, information is to be provided to the Chairman who may then decide the proper procedure of refurbishing the lost/damaged book.

SOP Computer lab:

1. Before using the computers, users have to make an entry in the log register.

2. The users are not allowed to install any unauthorized software.

3. The user will be held responsible for any physical damage to the computer.

4. The user should not delete/uninstall any data or software.

5. Proper decorum is observed. Eating and drinking are not allowed.

6. Other precautions like dusting, cleaning, proper hygiene are regularly undertaken.

Fetal Lab

Department of Anatomy, JNMC, AMU

SOP:

• Ethical clearance has been obtained from Institutional Ethical Committee (IEC) for fetal lab.

• Procured fetuses are brought after full parental consent.

• They are observed for gross anomalies and photographed.

• Records of each fetus with gross finding are kept separately for further correlation and research.

• Then they are fixed with 10% formalin using standard established protocol.

• After fixation they are glass-mounted and kept in formalin glass jars.

• Wherever any research project/PG theses (intradepartmental or interdepartmental) involving fetuses is planned (after due ethical clearance), they are allowed access.

• Research works related to gross fetal anatomy and histology are carried out.

• All observations are recorded, discussed, published and used for improving fetal outcome and well being.

Facilities available:

• Embalming of fetuses.

• Jars to keep embalmed/fixed fetuses.

• Formalin.

Total number of glass mounted fetuses - 90.

Neuroanatomy Laboratory

Department of Anatomy, JNMC, AMU

SOP:

       - Matter is fully explained and agreed upon to work with the limited facilities.

       - Minor surgical procedures are performed as per approval by IAEC, JNMC.

       - Tissue samples intended for further study are procured and stored.

       - Decalcification of skeletal tissue is also performed as per requirement.

       - Tissue is processed for microtomy, usually with the help of rotary microtome.

       - Paraffin sections are stained with H & E for histopathological study.

-  Special staining for nervous tissue and connective tissues are also performed.

-  Findings are recorded from the permanent mount of stained tissue sections.

Facilities available for:

         - Minor surgical / experimental procedures.

         - Procurement / collection of tissue samples.

         - Tissue processing - embedding and sectioning.

         - Staining- both routine and special for microscopic studies of specimens.

Staff:

       - Faculty / researcher / collaborator in the research project himself / herself.

       - No regular technical staff or laboratory attendant for assistance or maintenance.

       - MTS of the Department clean the laboratory on regular basis.

Beneficiaries:

- 02 PhDs in Anatomy (full work).

- 02 PhDs from other fields (partial work).

- 12 MDs (thesis work from Anatomy and other subjects).

- 12 Research scholars from other departments / disciplines.

- 01 Research project, (ICMR-funded) completed.

Outcome:

- Over 100 publications from research collaboration.

- Some interesting slides for teaching purposes.

Embryology and Birth Defect Laboratory

Department of Anatomy, JNMC, AMU

SOP:

• Tissues are stored and processed for freezing microtomy.

• Processed tissues undergo freezing microtomy.

• Sections are stained for histopathological studies.

• Mounted slides are then seen under the microscope.

Scope and Purpose:

• Histological processing using fixation and heat may inactivate antigens and genetic material within the tissue, therefore frozen sections must be used.

• This procedure is used for the preparation of interval sections for staining and gene sequencing.

Staff:

• Faculty and Post Graduate students .

• Existing technical staff of the department (no exclusive staff for the lab).

• MTS of the department clean the laboratory on regular basis.

Instruments Available:

1. Freezing microtome

2. Incubator

3. Slide warmer

4. Freeze

Chemicals:

Xylene	Phenol crystal	9. Sulphuric acid
Diethyl ether	DPX mountant	10. Dioxan
Chloroform	Acetone extrapure	11. Methanol
Propanol	Toludene	12. Ethyl alcohol

OFFICE

STANDERD OPERATING PROCEDURE

Name of Office/Department	ANATOMY
Chairperson	PROF. FARHAN KIRMANI
Contact No.	0571-2720406 & 7035, 7041 (internal numbers)
Email Address	anatomyjnmcamu@gmail.com
Web address (AMU)	amu.ac.in

STAFF		RESPONSIBILITIES
Mohd. Shahzad Khan Assistant (Admin.)		Performing administrative and routine clerical tasks including organizing and managing files, scheduling meetings and supporting other staff with organizational tasks.
Imran Ali (DW) Sanowar Ali (DW)		Receive & Dispatch, Diary of internal and external Dak, Maintenance of receipt and Dispatch register, Operating Xerox Machine etc. and other works as assigned by the Chairperson
WORK & RECORD MANAGEMENT		Evaluating and helping to develop system to support the efficient access, movement, cataloging, updating, storage, retention and disposal of files and other records.
		Managing and registering records, for example ensuring appropriate processing of digital records.
		Establishing procedures for how long records should be maintained or kept.

                     

EQUIPMENTS

Desktop Computers, UPS, Scanner, Xerox Machine, Telephone, Water Purifier etc.

TIMINGS

08:00 AM to 04:00 PM (All Working Days except Fridays) 08:00 AM to 12:30 PM (on Fridays)