Dr. Afzal Husain

    Ph.D. (Medicine), M. Sc. (Biotechnology), B.Sc.(Hons) Chemistry


    Assistant Professor


    Genome Instability and Integrity


    Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002





    MSc SEM I and SEM III: SESSION 2021-22BSc SEM V: SESSION 2021-22


Dr. Afzal Husain has done his Ph.D. with Prof Tomoyoshi Nozaki at Gunma University and the National Institute of Infectious Diseases, Japan. In Ph.D., he applied metabolomic, transcriptomic, enzymological approaches to discover novel metabolic pathways for developing chemotherapeutic agents against human protozoan parasites. He then spent several years with Nobel Laureate Prof. Tasuku Honjo at Kyoto University, Japan. He applied chromatin proteomics and reverse genetic approaches to study the mechanisms of activation-induced cytidine deaminase (AID)-dependent antibody gene diversification as well as genomic instability associated with B-cell lymphomas. After completing his postdoctoral research, he worked as Research & Development Manager at Thermo Fisher Scientific, Bengaluru, where he led Epigenetics and Custom Antibody Development Teams to develop and validate recombinant rabbit antibodies against drugs, proteins, and post-translational modifications. Dr. Husain has published his research in top-tier international journals, including EMBO Journal, Nature Communication, Proceedings of the National Academy of Sciences, and Journal of Biological Chemistry. Currently, his research focuses on understanding the mechanisms of genome instability.

  1. Publications

    Begum NA, Haque F, Stanlie A, Husain A, Mondal S, Nakata M, Taniguchi T, Hisaaki T, Tasuku Honjo. Phf5a regulates DNA repair in class switch recombination via p400 and histone H2A variant deposition. EMBO J. 2021 Jun 15;40(12):e106393.

    Husain A*, Begum NA, Kobayashi M, Honjo T. Native co-immunoprecipitation assay to identify interacting partners of chromatin-associated proteins in mammalian cells. Bio-protocol. 10(23): 2020. e3837.

    Husain A, Xu J, Fujii H, Kobayashi M, Nakata M, Wang Ji-Yang, Rehwinkel J, Honjo T, Begum NA. SAMHD1-mediated dNTP degradation is required for efficient DNA repair during antibody class switch recombination. EMBO Journal. 2020 Aug 3;39(15): e102931.

    Azza AI, Husain A, and Honjo T et al. Depletion of recombination-specific cofactors by the C-terminal mutant of the activation-induced cytidine deaminase causes the dominant negative effect on class switch recombination.International Immunology, 2017, 29, Issue 11,525–537.

    Husain A, and Tasuku Honjo et al, et al. Chromatin remodeller SMARCA4 recruits topoisomerase 1 and suppresses transcription-associated genomic instability. Nature Communications. 2016 Feb 4; 7:10549.

    Xu J, Husain A, and Honjo T et al. APE1 is dispensable for S-region cleavage but required for its repair in class switch recombination. Proceedings of Natural Academy of Sciences. 2014 Dec 2;111(48):17242-7.

    Jeelani G, Husain A, Nozaki T et al. Biochemical and functional characterization of novel NADH kinase in the enteric protozoan parasite Entamoeba histolytica. Biochimie. 2013 Feb;95(2):309-19.

    Husain A, and Nozaki T et al. Dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite Entamoeba histolytica. PLoS Neglected Tropical Diseases. 2012;6(9): e1831.

    Penuliar GM, Furukawa A, Nakada-Tsukui K, Husain A, and Nozaki T, et al. Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba histolytica. Journal of Antimicrobial Chemotherapy. 2012 Feb;67(2):375-86.

    Jeelani G, Sato D, Husain A, Nozaki T et al. Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation. PLoS One. 2012;7(5): e37740.

    Husain A, Jeelani G, Sato D, and Nozaki T et al. Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica. BMC Genomics. 2011 May 31; 12:275.

    Husain A, and Nozaki T. et al. Metabolome analysis revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica. Journal of Biological Chemistry. 2010 Dec 10;285(50):39160-70.

     Husain A, Jeelani G, Sato D, Ali V, Nozaki T Characterization of two isotypes of l-threonine dehydratase from Entamoeba histolytica. Molecular and Biochemical Parasitology. 2010 Apr;170(2):100-4.

    Jeelani G, Husain A, Nozaki T et al. Two atypical L-cysteine-regulated NADPH-dependent oxidoreductases involved in redox maintenance, L-cystine and iron reduction, and metronidazole activation in the enteric protozoan Entamoeba histolytica. Journal of Biological Chemistry. 2010 Aug 27;285(35):26889-99.

    Nakada-Tsukui K, Saito-Nakano Y, Husain A, Nozaki T et al. Conservation and function of Rab small GTPases in Entamoeba: annotation of E invadens Rab and its use for the understanding of Entamoeba biology. Experimental Parasitology. 2010 Nov;126(3):337-47.