DEPARTMENT.FACULTY

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Dr. Shariq Ahmed
  • DEPARTMENT_STAFF.QUALIFICATION

    MD

  • DEPARTMENT_STAFF.DESIGNATION

    Assistant Professor

  • DEPARTMENT_STAFF.THRUST_AREA

    Viriology, Mycobacteriology, Mycology, Immunology

  • DEPARTMENT_STAFF.ADDRESS

  • DEPARTMENT_STAFF.MOBILE

  • DEPARTMENT_STAFF.EMAIL

    shariqahmed0105@gmail.com

DEPARTMENT_STAFF.COMPLETE_CV

Dr. Shaiq Ahmed is working as an Assistant Professor in the Department of Microbiology, J.N.M.C., A.M.U., Aligarh.  He joined the institute in 2014 as a junior resident and completed his MD microbiology in 2017. After that, he did his SRship from 2017-2020. He is actively involved in post-graduate and undergraduate teaching-learning activities. His field of interest are Mycobacteriology, Virology, COVID-19, Mycology, Immunology and Hospital Acquired Infections.  

  1. Gut resistome with special reference to beta-lactamase-producers from human, poultry, and cattle from North-Indian region: a step towards “One Health” approach

    ABSTRACT

    Background: There is lack of systematic studies simultaneously comparing antibiotics resistance, and related genes (ARGs) from

    gut of animals and humans, and subsequently comparison with clinical isolates.

    Methods: 137 Gram-negative bacteria from gut of poultry, cattle, and healthy human volunteers and a subset of 74 GNB were

    studied for frequency/patterns for antibiotics resistance, and prevalent ARGs (blaCTX-M, blaTEM, blaSHV, blaampC and blaNDM-1) by PCR.

    Comparative analyses for resistance rates and patterns, and existing genes were done. Representative PCR amplicons were

    sequenced and analysed for precise bla type and RAPD typing of the human faecal and clinical isolates was done to see any

    clonal relatedness/diversity.

    Results: Varying frequency of resistance was noticed in gut isolates from poultry, cattle and healthy human volunteers and the

    patterns were different. Resistance rates were much higher in clinical isolates than the gut flora, including from healthy human

    volunteers. No resistance was seen with colistin in neither clinical nor gut isolates from poultry, cattle, and healthy human

    volunteers. Resistance to minocycline and tigecycline was noticed in 28.78% and 30.30% clinal isolates, respectively. From 139

    faecal isolates, a total of 7 CTX-M (5.03%; 7/139), 10 TEM (7.19%; 10/139), and 4 SHV (2.87%; 4/139) were detected. CTX-M was

    more prevalent in the human gut isolates (13.89%; 5/36) as compared to poultry (1.69%; 1/59) and cattle (2.27%; 1/44), whereas

    TEM was found to be more prevalent in poultry isolates (13.56%; 8/59). On the other hand, AmpC was present in significant

    proportion (58.27%; 81/139) of the gut isolates from all the three test groups and was almost equally distributed with the highest

    occurrence in cattle. Among clinical isolates, maximum occurrence of bla genes was of blaCTX-M (58%), followed by blaampC

    (40%), blaSHV (26%), and blaTEM (12%). Sequencing of representative isolates showed presence of CTX-M-15, TEM-1 and

    SHV-38. There was no clonal relatedness between human faecal isolates and clinical isolates.

    Conclusions: Multi-drug-resistance of varying frequency was noticed in faecal isolates and patterns were different between faecal

    and clinical isolates. There appears to be frequent and wider dissemination of class C beta-lactamase (AmpC) at animal and

    human interface, however wider dissemination of class A ESBL (CTX-M, TEM, SHV) has not yet established. Though no

    resistance to colistin in animal- and human-faecal and clinical isolates is a sign of relief, appearance of resistance to reserved drug

    such as tigecycline is alarming.

    Keywords: gut resistome; antibiotics resistance genes; poultry; cattle, healthy human volunteer; clinical bacterial isolates;

    comparative study; India


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  2. Profile of drug?resistant?conferring mutations among new and previously treated pulmonary tuberculosis cases from Aligarh region of Northern India
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  3. Evaluation of genotype MTBDRplus line probe assay in detection of rifampicin and isoniazid resistance in comparison to solid culture drug susceptibility testing in a tertiary care centre of western Uttar Pradesh
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LISTDownloadUPLOADED DATE
Acute pyelonephritis
11/10/2021