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Rajiv Gandhi Centre for Diabetes and Endocrinology

Research

Research

12/03/2021

Ph.D. Research Topic.

1.Studies on glycated albumin: Assessment of optimal cut off value of glycated albumin and its clinical relevance in diabetes and diabetes associated pathologies  by Mr. Alok Raghav; Supervisor Prof. Jamal Ahmad (AWARDED)

2. A clinic-microbial study of diabetic foot infections: Conventional and molecular approaches for bacterial identifications and quantifications by Miss. Saba Noor; Supervisor Prof. Jamal Ahmad.  (AWARDED) 

3. Role of Chromium, Melatonin and oxidative stress in type II diabetes by Mr. Doddigarla Zephy, Supervisor; Prof. Jamal Ahmad. (AWARDED) 

4. Amdori albumin in diabetic patients with chronic kidney disease: DNA profiling of the patients and healthy subjects by  Ms. Neelofar; Supervisor Prof. Jamal Ahmad. (AWARDED)

5.     Epigenetic & Genomic Study of Candidate Gene Promoter in North Indian Type 2 Diabetic  Nephropathy Subjects by Amit Mukherjee. Supervisor :   Prof. Sheelu Shafiq Siddiqi, Rajiv Gandhi Centre for Diabetes & Endocrinology, Faculty of Medicine, JNMC, AMU, Aligarh.


Co-Supervisor:   Professor. B.D. Banerjee, Department of Biochemistry, University College of Medical Sciences, University of Delhi, DELHI 

(ONGOING)




         





Program Educational Objectives

12/03/2021

Rajiv Gandhi Centre for Diabetes and Endocrinology, Faculty of Medicine, J.N. Medical College, Aligarh Muslim University, Aligarh


Syllabus for Paper I (Ph.D. Endocrinology)



Unit 1:       Research Methodology I (Research Planning & Literature Search)

  • Research Problem-conception, hypothesis, designing and implementaory

  • Use of search engine in literature retrieval, citation index, impact factor, H-index


Unit 2:       Research Methodology II (Cytological, Biochemical and Immunology Techniques)

  • Advances in Microscopy technique – LM, EM, Confocal microscopy, Karyotyping, Image analysis software.

  • Uses of HPLC, Amino acid analyser and Bomb calorimeter.



Unit 3:         Research Methodology III (Molecular Biology Techniques)

  • Basic concept of extraction and quantification of nucleic acid and proteins.

  • Proteins and nucleic acid Electrophoresis, Northern, Southern and Western bolts. FISH and CISH.

  • Protein and DNA band profiling through Gel documentation system.

  • PCR, cloning, sequencing, microarray.



Unit 4:       Statistical Analysis and use of computers in Research.

  • Significance of statistics in biological research.

  • Measure of central tendencies and deviation, test of significance.

  • Use of statistical software -  SPSS, Sigma plot, Statistical, Metlab.

  • Populations genetics analysis software -  Popgen, TFPGA, Arlequin.

  • Sequence analysis software -  BLAST, MultiAlign, BioWWdit, Clustal-W.



Unit 5:       Laboratory Techniques for Recognition of Endocrine Disorders:

  • Types of assays (Competitive immunoassays, immunometric assay, free hormone assays, hormatographic assays, mass spectrometry nucleic acid based assays.

  • Analytic validation (method comparison, precision, linearity, recovery, detection limit, stability, types of specimens)

  • Quality Assurance.



Syllabus for Paper II (Ph.D. Endocrinology Ongoing)




                                  

Unit 1:                   Diabetes : General concept

Types, complications, Epidemiology, Pathophysiology, b-cell function, mechanism of action of insulin, insulin resistance, dyslipidemia.


Unit 2:             Diabetic Nephropathy (DN)

Epidemiology, Pathogenesis, stages of Diabetic Nephropathy(DN), risk factors for developing DN.


Unit 3:             Recognition of Diabetic Nephropathy(DN) subjects

                        Conventional methods for identification of patients suffering from DN.


Unit 4:             Genetic approach for Diabetic Nephropathy (DN)

                       Genetics& epigenetics of Diabetic Nephropathy,Genome Wide Association Study (GWAS) in Diabetic Nephropathy, role of SNP’s in Diabetic Nephropathy.


Unit 5:             Review of Literature/Published research work

The candidate is expected to answer questions from the following research articles/review articles.


  • Recent progress in genetic and epigenetic research on type 2 diabetes, Experimental & Molecular Medicine (2016) 48, e220; doi:10.1038/emm.2016.7; published online 11 March 2016

  • The Genetics of Diabetic Nephropathy, Journal: Genes 2013, 4, 596-619; doi:10.3390/genes4040596

  • Genetics and Epigenetics of Diabetic Nephropathy, Journal: Kidney Diseases 2015;1:42–51, DOI: 10.1159/000381796, Received: March 8, 2015, Accepted after revision: March 20, 2015, Published online: April 16, 2015

  • Genetic Factors in Diabetic Nephropathy, Clin J Am SocNephrol2: 1306–1316, 2007. doi: 10.2215/CJN.02560607

  • Genetic and Epigenetic Aspects of Type 2 Diabetes Mellitus: A Review,Austin Endocrinol Diabetes Case Rep - Volume 1 Issue 1 - 2016